摘要
目的 探讨人参牛油果丸纳米制剂在抗衰老、心血管保护、中枢神经保护及改善糖尿病方面的综合功效。方法 通过斑马鱼模型，分别构建过氧化氢诱导的衰老模型、维拉帕米诱导的心衰模型、霉酚酸吗啉乙酯诱导的中枢神经损伤模型及高糖高脂饲料诱导的糖尿病模型，评价人参牛油果丸纳米制剂对各模型的干预效果。通过q-PCR检测衰老相关基因spns1表达，分析心输出量评估心功能，AO染色观察神经细胞凋亡，并测定血糖水平。结果 人参牛油果丸纳米制剂可显著上调衰老模型斑马鱼spns1基因表达（P<0.001），提升心衰模型心输出量（P<0.001），抑制中枢神经细胞凋亡（P<0.001），并降低糖尿病模型血糖水平（P<0.05）。在最大耐受浓度范围内，各模型均未出现明显毒性反应。结论 人参牛油果丸纳米制剂具有多靶点、多途径的协同作用，在抗衰老、心血管保护、神经保护及降血糖方面表现出显著功效，其作用机制与多种活性成分的协同效应及纳米技术提升生物利用度密切相关。
关键词： 中药复方纳米制剂；人参牛油果丸；抗衰老；心血管保护；中枢神经保护；糖尿病
Abstract
Objective To investigate the comprehensive effects of Ginseng and Avocado Pill Nanopreparation on anti-aging, cardiovascular protection, central nervous system protection, and improvement of diabetes. Methods Zebrafish models were established, including an H2O2-induced aging model, a verapamil-induced heart failure model, a mycophenolate mofetil-induced central nervous injury model, and a high-glucose/high-fat diet-induced diabetes model. The intervention effects of the Ginseng and Avocado Pill Nanopreparation were evaluated by detecting spns1 gene expression via q-PCR, analyzing cardiac output, observing neuronal apoptosis through AO staining, and measuring blood glucose levels. Results The nanopreparation significantly up-regulated spns1 gene expression in the aging model (P<0.001), improved cardiac output in the heart failure model (P<0.001), inhibited neuronal apoptosis in the central nervous injury model (P<0.001), and reduced blood glucose levels in the diabetes model (P<0.05). No significant toxic reactions were observed within the maximum tolerated concentration range. Conclusion The Ginseng and Avocado Pill Nanopreparation exhibits multi-target and multi-pathway synergistic effects, demonstrating significant efficacy in anti-aging, cardiovascular protection, neuroprotection, and hypoglycemic activity. Its mechanism is closely associated with the synergistic effects of multiple active components and enhanced bioavailability via nanotechnology.
Key words： Traditional Chinese medicine compound nanopreparation; Ginseng and avocado pill; Anti-aging; Cardiovascular protection; Central nervous system protection; Diabetes
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