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当前位置：国际中医药研究 > 2024年 4卷 (3)期

Open Access Article

International Research in Chinese Medicine. 2024; 4: (3) ; 10-13 ; DOI: 10.12208/j.ircm.20240044.

Study on the inhibitory effect of Shaoyao Ruangan prescription on HepG2 proliferation based on Lin28a/let-7a axis
基于Lin28a/let-7a轴探讨芍药软肝方抑制HepG2增值作用的研究

作者： 王鑫昱 *, 马永力, 刘立权, 文雯

浙江中医药大学附属杭州市中医院浙江杭州

*通讯作者：王鑫昱,单位：浙江中医药大学附属杭州市中医院浙江杭州；

杭州市医药卫生科技项目（A20200020）

发布时间: 2024-09-26 总浏览量: 49

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摘要

目的研究芍药软肝方对肝癌细胞HepG2增值抑制的影响及初步作用机制。方法 MTT法检测不同浓度芍药软肝方对HepG2细胞增殖的影响；qRT-PCR和Western blot检测芍药软肝方对Lin28a/let-7a轴的影响。结果芍药软肝方能够抑制HepG2细胞增殖，并诱导其凋亡；芍药软肝方能够上调let-7a表达，下调Lin28a表达。结论芍药软肝方可能通过调控Lin28a/let-7a轴抑制HepG2增值，诱导细胞凋亡，为肝癌治疗提供新的思路。

关键词： 芍药软肝方；肝癌；细胞凋亡；Lin28a；let-7a

Abstract

Objective To study the effect of Shaoyao Ruangan prescription on hepatocellular carcinoma cell HepG2 proliferation inhibition and its preliminary mechanism.
Methods MTT assay was used to detect the effect of Paeonia Ruangan Decoction on the proliferation of HepG2 cells. The effect of Shaoyao Ruangan prescription on Lin28a/let-7a axis was determined by qRT-PCR and Western blot.
Results Shaoyao Ruangan Formula could inhibit proliferation and induce apoptosis of HepG2 cells. Shaoyao Ruangan formula could up-regulate the expression of let-7a and down-regulate the expression of Lin28a.
Conclusion Shaoyao Ruangan formula may inhibit HepG2 proliferation and induce apoptosis by regulating Lin28a/let-7a axis, providing a new idea for the treatment of liver cancer.

Key words： Shaoyao Ruangan formula; Liver cancer; Cell apoptosis; Lin28a; let-7a

参考文献 References

[1] Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018, 68(6): 394-424.

[2] Llovet JM, Zucman-Rossi J, Pikarsky E, et al. Hepatocellular carcinoma[J]. Nat Rev Dis Primers, 2016, 2: 16018.

[3] Forner A, Reig M, Bruix J. Hepatocellular carcinoma[J]. Lancet, 2018, 391(10127): 1301-1314.

[4] Wong VK, Tse SK, Lam KC, et al. Chinese medicine for the treatment of hepatocellular carcinoma: a systematic review and meta-analysis of randomized controlled trials[J]. Integr Cancer Ther, 2014, 13(1): 1-12.

[5] 姜建伟,钟晓明,章红燕,等.芍药软肝方对肺癌化疗患者肝损伤的保护作用[J].中国中医药科技,2019,26(04): 598 -600.

[6] 孙燕,张爱琴,余新民,等.芍药软肝合剂对肝癌H_(22)荷瘤小鼠的抑瘤作用及对Bax、Survivin表达的影响[J].浙江中医杂志,2013,48(11):839-841.

[7] 李骏飞,郑小蓉,姜建伟,等.基于Bax/Bcl-2-Caspase-3通路芍药软肝方抑制裸鼠肝癌生长及机制研究[J].中国现代医生,2021,59(07):38-41+45+193.

[8] Viswanathan SR, Daley GQ. Lin28: A microRNA regulator with a macro role in development and disease[J]. Cell, 2010, 140(4): 445-449.

[9] Boyerinas B, Park SM, Hau A, et al. The role of let-7 in cell differentiation and tumor suppression[J]. Endocr Relat Cancer, 2010, 17(1): F19-F36.

[10] Heo I, Joo C, Cho J, et al. Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA[J]. Mol Cell, 2008, 32(2): 276-284.

[11] Balzeau J, Menezes MR, Cao S, Hagan JP. The LIN28/let-7 Pathway in Cancer. Front Genet. 2017 Mar 28;8:31. doi: 10.3389/fgene.2017.00031.

[12] Li G, Wakao S, Kitada M, Dezawa M. Tumor suppressor let-7 acts as a key regulator for pluripotency gene expression in Muse cells. Cell Mol Life Sci. 2024 Jan 23;81(1):54. doi: 10.1007/s00018-023-05089-9.

[13] Deshmukh SK, Srivastava SK, Zubair H, Khan MA, Singh AP, Singh S. Resistin Induces LIN28A-Mediated Let-7a Repression in Breast Cancer Cells Leading to IL-6 and STAT3 Upregulation. Cancers (Basel). 2021 Sep 7;13(18):4498.

引用本文

王鑫昱, 马永力, 刘立权, 文雯, 基于Lin28a/let-7a轴探讨芍药软肝方抑制HepG2增值作用的研究[J]. 国际中医药研究, 2024; 4: (3) : 10-13.

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