摘要
目的 探讨染料木素对双氧水诱导的PC12细胞炎症模型的影响及其抗炎作用机制。方法 1、筛选适合造模的双氧水浓度构建PC12细胞炎症模型。2、测定不同浓度染料木素对细胞存活率的影响。3、MTT法观测染料木素对PC12细胞双氧水炎症模型的预保护作用。4、实时PCR检测各组PC12细胞中NgR mRNA的表达。结果 1、染料木素浓度100 μM以下基本不具细胞毒性，染料木素预处理具有保护作用，与模型组相比显著提高PC12炎症模型细胞存活率。2、染料木素显著下调H2O2损伤引起NgR基因的表达。结论 染料木素可通过Nogo/NgR信号通路，下调NgR水平发挥抗PC12神经细胞炎症的作用。
关键词： 染料木素；神经炎症；抗炎；NgR
Abstract
Objective To To investigate the effect of genistein on PC12 cell inflammatory model induced by hydrogen peroxide and its anti-inflammatory mechanism. Methods 1. Select the suitable concentration of hydrogen peroxide for modeling to construct PC12 cell inflammation model. 2. The effects of different concentrations of genistein on cell survival were determined. 3. MTT assay was used to observe the protective effect of genistein on PC12 cells with H2O2 induced inflammation. 4. The expression of NgR mRNA in PC12 cells of each group was detected by real-time PCR. Result 1. Genistein concentration 100 μ There is basically no cytotoxicity below M, and genistein pretreatment has a protective effect. Compared with the model group, it significantly improves the cell survival rate of PC12 inflammatory model. 2. Genistein significantly decreased the expression of NgR gene induced by H2O2 damage. Conclusion Genistein can inhibit the inflammation of PC12 neurons by down regulating the level of NgR through Nogo/NgR signaling pathway.
Key words： genistein; Neuroinflammation; Anti inflammation; NgR
参考文献 References
[1] 王雪,代朝,方坚松.刺五加防治神经退行性疾病的药理研究进展[J].中国中药杂志,2022,10(05),1-9.
[2] 肖飞. Nogo-66受体作为阿尔茨海默病药物治疗新靶标的研究[D].广州:暨南大学,2010.
[3] 孔繁格.连翘酯苷B对阿尔兹海默症的保护作用及其相关机制研究[D].长春:吉林大学,2020.
[4] ZHOU R, JI B,KONG Y, et al. PET imaging of neuroinflammation in Alzheimer's Disease[J]. Front Immunol, 2021;12:739130.
[5] ALREHAILI AA, LEE JY, BAKHURAYSAH MM, et al. Nogo receptor expression in microglia/macrophages during experimental autoimmune encephalomyelitis progression[J]. Neural Regen Res, 2018,13(5): 896-907.
[6] 刘百峰,王晓芳,徐行,等.NgR特异性siRNA筛选及其慢病毒表达载体构建[J].中国伤残医学, 2013,21(07):99-101.
[7] 崔佳莹,许峰巍,彭莹莹,等.染料木黄酮抗肿瘤作用研究进展[J].药物评价研究,2018,41(12): 2369-2375.
[8] 黄河,管家宁,冯爱露,等.染料木素对阿霉素诱导心脏毒性保护作用的初步研究[J].徐州医科大学学报,2021,41(12):859-864.
[9] 朱卫丰,李佳莉,孟晓伟,等.葛属植物的化学成分及药理活性研究进展[J].中国中药杂志,2021, 46(06):1311-1331.
[10] 徐梦蕾.大豆异黄酮对甲萘威诱导损伤的PC12细胞的保护作用[D].长春:吉林大学,2016.
[11] HONG H, LIU GQ. Protection against hydrogen peroxide-induced cytotoxicity in PC12 cells by scutellarin[J]. Life Sci, 2004,74(24):2959-2973.